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KMID : 1149220110190010195
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Korean Journal of Oriental Medical Prescription
2011 Volume.19 No. 1 p.195 ~ p.205
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( Anti-inflammatory effect of ethanol extract from Ecklomia cava on gram-positive bacteria(Peptidoglycan)-induced macrophages
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Kang Ok-Hwa
Kim Sung-Bae
Keum Joon-Ho
Mun Su-Hyun
Kim Yong-Sik
An Byung-Kwan
An Hyeon-Jin
Kwon Dong-Yeul
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Abstract
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Objectives:Ecklonia cava is brown alga(Laminariaceae) which grows is sea, it has antioxidant, diarrhea and anticoagulant effect. In this study, the effect of ethanol extract of Ecklonia cava (EC) on peptidoglycan(PGN) -induced NO production in RAW 264.7 cells was investigated.
Methods:In the present study, IL-6 production was measured using the enzyme-linked immunosorbent assay(ELISA), prostaglandin [＼E?]₂([＼PGE?]₂) production was measured using the EIA kit, and inducible NO synthase(iNOS), cyclooxygenase-2(COX-2), and mitogen-activated protein kinase(MAPK) activation, as determined by western blot analysis and reverse transcription -polymerase chain reaction(RT-PCR).
Results: EC inhibited PGN-induced NO and IL-6 production. Consistent with these observations, the protein expression of iNOS and COX-2 were inhibited by EC. Moreover, EC suppressed the phosphorylation of extracellular signal-regulated kinase(ERK) 1/2 in PGN-induced RAW 264.7.
Conclusions :These results suggest that EC has inhibitory effects on PGN-induced [＼PGE?]₂, NO, and IL-6 production, as well as the expressions of iNOS and COX-2 in the murine macrophage. These inhibitory effects occur through blockades on the MAPKs phosphorylation.
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KEYWORD
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Ethanol extract of Ecklonia cava(EC), gram-positive bacteria(Peptidoglycan), anti-inflammation, Macrophages
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